As reported previously, some types of glass vials can lead to in-vial chemical changes of the API. 1–5 Wallace notes that even small quantities of particulate matter on the surface of the vials can have an effect on data quality, including but not limited to irreproducible results or sample loss due to adsorption. 2 Arvary and Mangion
Sep 5, 2014 · Indeed, proper solubilization is one of the most important factors for a successful analysis. In addition, alternative approaches are described to improve analyte recovery from the sample vial. The second part focuses on strategies to efficiently reduce adsorption at components of the autosampler, column and mass spectrometer.
This report provides important financial rationale and regulatory justification for manufacturers of priority essential medicines to maintain a robust CV program and conduct a risk mitigation strategy for minimizing cross-contamination. It includes a case study and examples that demonstrate how to
the vial is left undisturbed without vortexing, there is continuous adsorptive loss with time, but most of the loss takes place in the first 20 minutes (Figure 2). In typical lab workflow, the sample solution has been in contact with the glass surface for a few hours before the assay. Analyte adsorption to glass vials may be completed. Time
Oct 11, 2018 · Instrument effects—principally carryover—are the most common source of ghost peaks. Carryover, particularly in this age of autoinjectors and highly parallel experimentation, may occur when sample components adhere to or adsorb onto the outside of the needle that aspirates sample from a vial or microwell.
1. Carryover – mostly due to analyte/sample matrix adsorption onto metal or plastic surfaces within the autosampler - Worn parts, either through damage or a rough surface, are of benefit to carryover as the sample matrix easily attaches to it. For example, needle outer surface versus the inner surface of the needle seat
Apr 11, 2016 · 3. Research the lab consumable you are using: Make sure lab consumables are made from high-quality plastic resins that contain low amounts of additives. Such resins are used to make chromatography
Jun 22, 2020 · The larger the expansion coefficient number, the greater the adsorption of analytes. Additional surface imperfections on the glass surface, such as scratches or holes, can increase surface adsorption. The time that that sample is present in the vial will also contribute to the degree of analyte adsorption. To study the effect of glass quality
Jun 2, 2020 · Ideally, they should have a low surface adsorption capacity to minimize analyte loss or adsorption onto the vial walls. Sample vials made from inert materials, such as borosilicate glass or high-quality plastics like polypropylene, are commonly used to minimize interactions between the sample and the vial surface.
Oct 1, 2006 · Carryover is the appearance of an analyte in a run when a blank containing no analyte is injected. This is illustrated in Figure 1 (a), where a peak corresponding to 1% of the sample peak is seen with the injection of a blank. Carryover is a problem, from a quantitative standpoint, only when the carryover peak is large enough to compromise the
Sample carryover is an issue for LC, especially LC/MS analysis, and can lead to reduced sample throughput and impact data quality. The causes of carryover are often in the autosampler, where the injection takes place1. To avoid, or at least reduce carryover from one sample to the next, it is important to clean the inside
Jun 20, 2023 · The small volume capacity of these vials, coupled with micro vial inserts, allows for the analysis of minute sample sizes, minimizing the need for large sample volumes while maintaining analytical sensitivity. The precise design and sealing properties of micro insert vials prevent sample loss, evaporation, and contamination, preserving the
Sep 5, 2014 · To test the extent of adsorption, a number of consecutive transfers of a sample from one vial to another can be performed, followed by the quantification of the analyte in each vial [38], [41], [42]. It is established that the aspecific adsorption of an analyte to a surface strongly depends on the equilibrium constant between solvent and surface.
The reduction or elimination of sample carryover and cross-contamination has become a major challenge in LC and LC/MS analysis. The Aijiren Infi nity II Multisampler is optimized for lowest carryover by careful design of the fl ow path and use of materials that minimize sample adsorption. Method settings of the Multisampler
Adsorption to the commercially available glass vial is conjectured to be more likely to occur because a greater surface area is contactable by the sample. In the future, I aim to perform further chemical analysis of the vial surface and further determine the mechanism by which adsorption is reduced.