• Protects against sample cross-contamination • Protects pipetter aid • Can slow fast-volume draw • Prevents liquid contamination in over-pipetting • Designed & certified for purity* Lower product costs • Reduces need for internal syringe filters • Enables laboratory engineering and work practice controls
Aug 25, 2022 · Cross-contamination of biological samples during handling and preparation, is a major issue in laboratory setups, leading to false-positives or false-negatives. Sample carryover residue in pipette
of sample-to-sample contamination. The action of pipetting can create aerosols or liquid pass-through that are drawn into the pipette and then transferred to subsequent samples, creating carryover contamination. Carryover and cross-contamination increase the risk of erroneous results, causing costly delays requiring laborious troubleshooting.
Cross-contamination in biological science is when part of one sample is transferred into another sample. Cross-contamination in medicine is when a pathogen is transferred from one individual to another. Final product contamination in the pharmaceutical industry is the product in a version the customer would use has been contaminated with a drug
Nov 10, 2022 · Possible sources of ghost peaks and carryover include: Sample preparation. Extra peaks can be present due to sample preparation: Possible source of contamination. Solution. Low quality or contaminated solvent. Check to see if the problems coincided with a change in solvent supply. If they did, change to a known contamination free solvent supply.
Alternatively, the concentration can be increased to 0.04 U/µl and the pre-incubation step omitted. Cod UNG will efficiently remove carry-over contamination during sample setup and cycler ramping. Here we show that Cod UNG can be used for carry-over prevention in a commercial one-step RT-qPCR master mix containing dUTPs instead of dTTPs.
Jun 20, 2023 · HPLC inserts play a crucial role in preserving the sample integrity by minimizing the contact between the sample and the vial surface. This prevents analyte adsorption, degradation, and contamination, ensuring reliable and consistent results.
"Carry-over" is a term used to describe a type of sample contamination which causes sample peaks to re-appear in later runs which do not actually contain the sample (e.g. blank runs). The
improved carryover mitigation in LC applications. Carryover has many sources, but the most common source of carryover is related to the autosampler and occurs when sample components adhere to or absorb onto the outside of the needle. A key attribute of modern autosamplers is the ability to effectivity remove sample from all active surfaces of the
Jan 20, 2022 · Unexpected patterns in contamination detection based on experimental set-up or proximity to samples with a high target concentration or reference materials can suggest sample cross-contamination (Huggett et al., 2020). Finally, if false-positives are observed in control reactions, it may be useful to sequence these samples to confirm that they
Nov 1, 2006 · Sample carryover was not observed for CK and albumin (< 5% difference in comparison to the reference values). Carryover was found to be minimal (≤ 0.2%). In an evaluation of the Abbott Architect c8000, Pauli et al. studied specimen carryover by testing 3 replicates of high concentration samples followed by nine replicates of low concentration
One of the major qPCR contamination sources is amplification carryover contamination. During the qPCR amplification process, millions of copies of the DNA template are produced. When you open a tube or plate containing the amplified product, significant quantities of the product can be aerosolized and easily dispersed into the lab environment.
Sample/Standard Carryover. I am running GC (FID detector) alongside G1888 headspace. I had noticed 4 of my later eluting peaks got increasingly worse in terms of shape and reproducibility. After running blanks (no injection and also blank vials), it seemed like the cause of this turns out to be carryover.
Apr 27, 2020 · The Consortium on Adventitious Agent Contamination in Biomanufacturing (CAACB) provides a comprehensive and forward-looking overview of industry’s experience with viral contamination of cell
Feb 28, 2015 · "Carry-over", Carryover, Carry over, HPLC, UHPLC, Injector, Contamination, is a term used to describe a type of sample contamination which causes sample peaks to re-appear in later runs which do not actually contain the sample (e.g. blank runs). The contamination can last for several sequential runs, often decreasing in amount after each injection (which is a key observation when troubleshooting).